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人胚肺細胞(MRC-5)
XG-X162188
MRC-5:MRC5
DMEM-H+10%FBS+1%P/S
貨號 純度 規格 目錄價 會員價 庫存 數量 購買
XG-X162188 1×106 1×106 ¥3000.00 登錄查看
產品詳情

細胞名稱:人胚肺細胞(STR鑒定正確)

細胞簡稱:MRC-5

產品貨號:TCH-C263

種屬來源:人

組織來源:肺

疾病特征:/

細胞形態:成纖維細胞樣

生長特性:貼壁生長

培養體系:DMEM-H+10%FBS+1%P/S

配套培養基貨號:TCH-G263

傳代比例:1:3-1:6,每2-3天換液一次

傳代周期:48-72 h

培養條件:氣相:95%空氣+5%CO2,溫度:37℃

凍存條件:60%基礎培養基+30%FBS+10%DMSO,液氮儲存

質量檢測:細菌、真菌、支原體檢測均為陰性

參考文獻:

Jacobs JP, et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953.

Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970.

Holliday R, Tarrant GM. Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262

Lewis CM, Tarrant GM. Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893

Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.

Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.

Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.

Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

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